Develops antibodies, recombinant proteins, peptides and molecular biology reagents for life science research
ABclonal, Inc.
Woburn, MA (USA)New on Amplifa
About ABclonal
ABclonal is a life science company headquartered in Woburn, Massachusetts (USA), that develops and distributes antibodies, recombinant proteins, peptides, ELISA kits and molecular biology reagents. The portfolio comprises more than 4,000 targets, over 5,000 catalog products and more than 10,000 clones, including recombinant rabbit monoclonal antibodies based on SMab™ technology (Single B-Cell platform), polyclonal and monoclonal antibodies, flow cytometry and IHC antibodies, and PTM-specific antibodies. Through its subsidiary Yurogen Biosystems, acquired in 2021, ABclonal offers custom antibody development and protein expression in bacterial, mammalian and yeast systems; Yurogen has completed more than 10,000 projects. The service offering also includes Custom Peptide Synthesis (up to 200 amino acids, from $1.98/amino acid, with MS and HPLC quality control by independent QA), peptide libraries, peptide modifications, and NGS raw material enzymes. Customers come from academic research, biotech and the pharmaceutical industry, including UCLA, Washington University in St. Louis and McGill University.
- Site
- Woburn, MA, USA
- Processes
- Recombinant monoclonal antibody development (SMab™ Single B-Cell platform), Monoclonal Antibody Development (Rabbit Monoclonal), Monoclonal Antibody Development (Mouse Monoclonal), Single Domain Antibody Development (Camelids/Nanobody), Polyclonal Antibody Production, PK and ADA Assay Reagent Development, Antibody Engineering and Characterization, Recombinant Protein Expression in E. coli (Bacterial System)
- Materials
- Peptides (amino acid chains up to 200 aa, incl. D-amino acids, N-methyl amino acids, non-natural, Carrier proteins for conjugation (KLH, BSA, Ovalbumin), PEG (Polyethylene Glycol), Enzymes: Taq DNA Polymerase (Thermus aquaticus YT-1, recombinant in E. coli), DNA Polymerase I, Large (Klenow) Fragment (E. coli polA), Klenow Fragment 3'→5' exo- (E. coli polA D355A,E357A), DNA Polymerase I (E. coli), DNase I, RNase-free
- Certificates
- Independent QA (MS, HPLC, AAA, Karl Fischer, MALDI-TOF MS, NMR)
- Industries
- Life Science Research (Academia), Biotech, Pharmaceutical Industry, Diagnostics/Therapeutics Development
- 01
Antigen design and antibody development (SMab™ Single B-Cell platform)
- 02
Antibody engineering and characterization
- 03
Recombinant protein expression (E. coli, mammalian, yeast)
- 04
Peptide synthesis (LPPS/SPPS, microwave technology)
- 05
Quality control (MS, HPLC, independent QA)
- 06
Sales and technical support
Products, Systems and Processes
SMab™ Technology (Single B-Cell Platform)
Platform for developing recombinant monoclonal antibodies by combining B-cell sorting, enrichment, bioinformatics and antibody expression.
Solid-Phase Peptide Synthesis (SPPS)
Used for longer peptides up to 200 amino acids and combined with microwave technology to increase coupling efficiency.
Liquid-Phase Peptide Synthesis (LPPS)
Used for short peptides to minimize side reactions.
Bacterial Expression System (E. coli)
Recombinant protein expression for structural biology, biochemical characterization and bioactivity assays.
Mammalian Expression System
Expression of recombinant proteins and recombinant antibodies in mammalian cell systems.
Yeast Expression System
Alternative recombinant protein expression in yeast systems.
NGS Raw Material Enzymes
Recombinant polymerases, ligases and nucleases (e.g. Taq DNA Polymerase, Klenow Fragment, T4 DNA Ligase) for PCR, RT-PCR and NGS applications.
Peptides incl. Special Amino Acids
Synthesis with D-amino acids, N-methyl amino acids and non-natural amino acids such as Orn, Abu, Aib, Nva, Nle, Hse, Hcy, Pen and Mpa.
Carrier Proteins (KLH, BSA, Ovalbumin)
Conjugation of peptides via cysteine residues to generate strong immune responses.
PEG (Polyethylene Glycol)
PEGylation improves the therapeutic applicability of macromolecules.
Biofuraw™ Laboratory Equipment
Own electrophoresis and gel imaging program with gels, power supplies (EPS-1500), electrophoresis cells (HE-90C, HE-120, HE-220) and imaging systems (MINI Space 2000/3000, Chemi Dog 5200T).
Locations
Woburn, MA (USA)
USSales, development and services (500W Cummings Park, Ste. 6500)
- Materials
- Antibodies, proteins, peptides, enzymes
- Chemi Dog 5200T Chemi-Image System
- MINI Space 2000/3000 Gel Imaging Systems
- EPS-1500 Power Supply
- HE-120/HE-220/HE-90C Electrophoresis Cells (Biofuraw™ catalog products)
Industries
Processes & technical limits
| Processes | Tolerance | Max. part | Weight | Lot size | Materials |
|---|---|---|---|---|---|
| Recombinant monoclonal antibody development (SMab™ Single B-Cell platform)Engineering | – | – | – | – | Rabbit, Camelids (Single Domain/Nanobody), Mouse, Human |
| Monoclonal Antibody Development (Rabbit Monoclonal)Engineering | – | – | – | – | Rabbit |
| Monoclonal Antibody Development (Mouse Monoclonal)Engineering | – | – | – | – | Mouse |
| Single Domain Antibody Development (Camelids/Nanobody)Engineering | – | – | – | – | Camelids, Llama |
| Polyclonal Antibody ProductionEngineering | – | – | – | – | Rabbit, Rat |
| PK and ADA Assay Reagent DevelopmentTesting | – | – | – | – | – |
| Antibody Engineering and CharacterizationEngineering | – | – | – | – | – |
| Recombinant Protein Expression in E. coli (Bacterial System)Engineering | – | – | – | – | E. coli |
| Recombinant Protein Expression in Mammalian SystemEngineering | – | – | – | – | Mammalian |
| Recombinant Protein Expression in Yeast SystemEngineering | – | – | – | – | Yeast |
| Recombinant Antibody Expression in Mammalian SystemEngineering | – | – | – | – | Mammalian |
| Peptide Synthesis (Liquid-Phase Synthesis LPPS for Short Peptides)Engineering | – | 200 amino acids | mg – kg | – | Amino acids/Peptides |
| Peptide Synthesis (Solid-Phase Synthesis SPPS for Longer Peptides, Microwave Technology)Engineering | – | 200 amino acids | mg – kg | – | Amino acids/Peptides |
| Peptide Libraries (Truncation, Positional, Alanine Scanning, Scrambled, Overlapping)Engineering | – | – | – | – | Peptides |
| Peptide Modifications (Acetylation, Amidation, Biotinylation, FITC/5-FAM Labeling)Engineering | – | – | Microgram (Isotope Labeling) | – | Peptides |
| Peptide Quality Control (MS, HPLC, AAA, Karl Fischer, MALDI-TOF MS, NMR)Testing | Purity up to 98% | – | – | – | Peptides |
7 Anlagen & Maschinen
| Machine | Manufacturer / model | Quantity | Working area | Year built | Site |
|---|---|---|---|---|---|
| Chemi-Image System | ABclonal (Biofuraw™) Chemi Dog 5200T | 1 (catalog | – | – | Woburn, MA |
| Gel Imaging System | ABclonal (Biofuraw™) MINI Space 2000 | 1 (catalog | – | – | Woburn, MA |
| Gel Imaging System | ABclonal (Biofuraw™) MINI Space 3000 | 1 (catalog | – | – | Woburn, MA |
| Power Supply (Electrophoresis) | ABclonal (Biofuraw™) EPS-1500 | 1 (catalog | – | – | Woburn, MA |
| Multi Horizontal Electrophoresis Cell | ABclonal (Biofuraw™) HE-120 | 1 (catalog | – | – | Woburn, MA |
| High-volume Horizontal Electrophoresis Cell | ABclonal (Biofuraw™) HE-220 | 1 (catalog | – | – | Woburn, MA |
| Mini Horizontal Gel Electrophoresis Cell | ABclonal (Biofuraw™) HE-90C | 1 (catalog | – | – | Woburn, MA |
Additional services
Reference customers
Quality
All peptides receive MS and HPLC validation reports through independent quality inspection; additional analyses include AAA, Karl Fischer water content, MALDI-TOF MS and NMR. Peptide synthesis is offered with a success rate above 95% and purities up to 98%.
| Evidence | Woburn, MA (USA) |
|---|---|
| Independent QA (MS, HPLC, AAA, Karl Fischer, MALDI-TOF MS, NMR) |
History
- 2021
Yurogen Biosystems became a subsidiary of ABclonal; the SMab™ technology was combined with ABclonal's life science portfolio.